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ADAP, which can be needed for up regulation of LFA 1 adhesion. This pathway is mediated downstream by SKAP1 that regulates the complex formation amongst Rap1 and RapL. Two tyrosine motifs at Y595DDV and Y651DDV of ADAP bind for the SH2 domain of OAC1 SLP 76 upon TCR stimulation. A double point mutation in ADAP at Y595F and Y651F is defective in SLP 76 binding and shows decreased LFA 1 adhesion and pSMAC formation. In spite of this, a possible connection amongst ADAP and HIV 1 infection has not been explored. Within this study, we demonstrate that ADAP and its bin ding to SLP 76 regulate two methods of HIV 1 infection by cooperating differentially with two distinct co receptors. Loss of ADAP and the SLP 76 ADAP module mar kedly impaired CD28 mediated HIV 1 transcription too as LFA 1 dependent formation of virological synapse for cell cell viral spread.
These findings iden tify ADAP and its signaling module as important regulators of HIV 1 infection. Outcomes Disruption Fer-1 the SLP 76 ADAP signaling module inhibits HIV 1 infection We and others have previously outlined the significance on the SLP 76 ADAP SKAP1 pathway within the activation of LFA 1. A mutant of ADAP lacking tyrosine resi dues 595 and 651 is unable to bind to SLP 76 and impairs LFA 1 activation. We assessed no matter if wild variety ADAP and the mutant M12 could regulate HIV 1 infection in Jurkat T cells. Jurkat T cells had been stably transduced with retroviral su pernatants encoding ADAP IRES GFP or M12 IRES GFP or with GFP alone. Expression remained steady because of inte gration.
The transfectants showed exactly the same expression levels Bafilomycin A1 of CD4, CXCR4, CD3, CD28, B1 and B2 integrins because the control GFP expressing Jurkat cells as measured by flow cytometry. RNA polymerase We next infected these cells with a single cycle HIV 1 virus carrying a luciferase reporter. The mRNA levels of HIV 1 gag had been measured at 72 hours post infection by quantitative RT PCR with precise primers for HIV 1 gag. JK ADAP GFP cells showed three 4 fold larger levels of HIV 1 gag mRNA when when compared with JK GFP cells. By contrast, JK M12 GFP cells failed to support the increase of HIV 1 gag mRNA beyond that observed within the JK GFP cells. The amount of transfected M12 was comparable to ADAP as seen by western blotting. We confirmed that after HIV 1 infection, overexpression of ADAP GFP or M12 GFP had no effects on CD4 or CXCR4 expression in Jurkat cells. We next stably overexpressed GFP, ADAP or M12 into human C8166 T cells.
These cells had been infected with low dose or higher dose of HIV 1. Superna tants had been collected and quantified by ELISA for levels of of HIV 1 p24Gag at several times post infection. We identified that at both doses of input Bafilomycin A1 virus, C8166 M12 cells had been impaired in their support of HIV 1 replication relative to cells expressing wild variety ADAP. When we utilised low dose of virus to infect cells, C8166 ADAP cells OAC1 and the control cells supported productive infection, whereas C8166 M12 cells failed to produce the detectable levels of p24Gag. More than 95% of C8166 T cells overexpressed GFP, or ADAP GFP or M12 GFP, which had no impact around the expression of surface receptors and showed comparable growth rates. We additional examined no matter if HIV 1 infection of human primary CD4 T cells was dependent on ADAP.
ADAP expression was decreased making use of precise siRNAs. qRT PCR showed a 50 60% reduction in ADAP mRNA transcripts over a period of 96 hours post transfection. Similarly, western blotting Bafilomycin A1 of cells at 48 OAC1 hours confirmed the signi ficantly decreased ADAP expression after transfection with siRNA ADAP. siRNA transfected human CD4 T cells had been then infected with the single cycle HIV 1 virus containing luciferase reporter. siRNA for ADAP decreased HIV 1 gag mRNA levels by 30% when assessed at 72 hours post infection. A measurement of luciferase activity confirmed that siRNA for ADAP resulted within a important reduction of HIV 1 infection. The surface expression of CD3, CD4, CD28, CXCR4, B1 B2 integrins and ICAM 1 in human CD4 T cells was not affected by knockdown of ADAP.
Collectively, Bafilomycin A1 these data indicate that ADAP is needed for the optimal HIV 1 infection of T cell lines and primary human T cells. ADAP and SLP 76 regulates HIV 1 LTR transcription within a CD28 and NFB dependent manner To uncover the molecular basis of ADAP involvement in HIV 1 infection, we firstly examined its possible ef fects around the induction of HIV 1 LTR transcription. Wild variety, SLP 76 deficient Jurkat T cells or ADAP deficient Jurkat T cells had been transfected with a pLTR gag3 flag luc reporter plasmid followed by stimulation by means of anti CD3 CD28 ligation for 6 hours. The pLTR gag3 flag luc plasmid includes the HIV 1 5 LTR promoter area with two NFB binding web sites and a firefly luciferase open reading frame. HIV 1 transcription was then assessed by a measure of luciferase activity. Anti CD3 CD28 stimula tion induced a two fold increase in HIV 1 transcription in wild variety Jurkat cells, an impact that was not seen in J14 cells. Re expression of SLP 76 into J14 cells restored and enhanced HIV 1 tran scription